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2de experiment  (ProteomeTech Inc)

 
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    ProteomeTech Inc 2de experiment
    Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis <t>(2DE)</t> analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.
    2de Experiment, supplied by ProteomeTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2de+experiment/pmc09598822-145-0-5?v=ProteomeTech+Inc
    Average 90 stars, based on 1 article reviews
    2de experiment - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Increased Susceptibility of Mycobacterium tuberculosis to Ethionamide by Expressing PPs-Induced Rv0560c"

    Article Title: Increased Susceptibility of Mycobacterium tuberculosis to Ethionamide by Expressing PPs-Induced Rv0560c

    Journal: Antibiotics

    doi: 10.3390/antibiotics11101349

    Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.
    Figure Legend Snippet: Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

    Techniques Used: Gene Expression, Two-Dimensional Gel Electrophoresis, Electrophoresis, Expressing, Microarray, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation



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    2de experiment  (ProteomeTech Inc)
    90
    ProteomeTech Inc 2de experiment
    Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis <t>(2DE)</t> analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.
    2de Experiment, supplied by ProteomeTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2de+experiment/pmc09598822-145-0-5?v=ProteomeTech+Inc
    Average 90 stars, based on 1 article reviews
    2de experiment - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

    Journal: Antibiotics

    Article Title: Increased Susceptibility of Mycobacterium tuberculosis to Ethionamide by Expressing PPs-Induced Rv0560c

    doi: 10.3390/antibiotics11101349

    Figure Lengend Snippet: Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

    Article Snippet: 2DE experiment was performed by ProteomeTech (Seoul, Korea) as previously described [ ].

    Techniques: Gene Expression, Two-Dimensional Gel Electrophoresis, Electrophoresis, Expressing, Microarray, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation